Overexpression of vacuolar H+‐ATPase‐related genes in bottom‐fermenting yeast enhances ethanol tolerance and fermentation rates during high‐gravity fermentation
作者： Sonoko Hasegawa , Tomoo Ogata , Koichi Tanaka , Akira Ando , Hiroshi Takagi
DOI: 10.1002/JIB.32
关键词: Fermentation 、 Ethanol 、 Lactic acid fermentation 、 Yeast 、 Biology 、 Ethanol fermentation 、 Brewing 、 Biochemistry 、 Industrial fermentation 、 Strain (chemistry)
摘要: Vacuolar H+-ATPase (V-ATPase) is thought to play a role in stress tolerance. In this study it was found that bottom-fermenting yeast strains, which the V-ATPase-related genes DBF2, VMA41/CYS4/NHS5 and RAV2 were overexpressed, exhibited stronger ethanol tolerance than parent strain showed increased fermentation rates high-sugar medium simulating high-gravity fermentation. Among strains examined, DBF2-overexpressing highest rate YPM20 medium. Using strain, performed by adding sugar wort, led viability compared with strain. These findings indicate V-ATPase target suggests enhancing activity increases of yeast, thereby improving cell under conditions. Copyright © 2012 The Institute Brewing & Distilling
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