Exon Array Analysis using re-defined probe sets results in reliable identification of alternatively spliced genes in non-small cell lung cancer

摘要: Treatment of non-small cell lung cancer with novel targeted therapies is a major unmet clinical need. Alternative splicing mechanism which generates diverse protein products and functional relevance in cancer. In this study, genome-wide analysis the alteration patterns between normal tissue was performed. We generated an exon array data set derived from matched pairs including both adenocarcinoma squamous carcinoma subtypes. An enhanced workflow developed to reliably detect differential set. total, 330 genes were found be differentially spliced compared tissue. Microarray findings validated independent laboratory methods for CLSTN1, FN1, KIAA1217, MYO18A, NCOR2, NUMB, SLK, SYNE2, TPM1, (in 10 events) ADD3, analysed depth. achieved high validation rate 69%. Evidence that activity FOX2, factor shown cause cancer-specific breast ovarian cancer, not altered at transcript level several types This study demonstrates how alternatively can identified Our underline key processes progression NSCLC are affected by alternative splicing, exploited search therapies.