Colorimetric Assay of Reitman and Frankel

摘要: Publisher Summary GOT (glutamate-oxaloacetate t ransaminase) activity can be measured colorimetrically by the use of two points. The first colorimetric assay this technique was described Tonhazy et al., in which reaction product oxaloacetate is quantitatively decarboxylated to pyruvate with aniline citrate as catalyst and then determined 2,4-dinitrophenylhydrazone. After a fixed time, 2,4-dinitrophenylhydrazone product, oxaloacetate, alkaline solution. During reaction, concentration 2-oxoglutarate decreases, while that increases. To prevent growth microorganisms solutions, containers need sterilized. NaOH (III) chromogen solution (II) are stable indefinitely if well stoppered. It not possible extinction coefficients 2,4-dinitrophenylhydrazones calculate results. All compounds form hydrazones 2,4-dinitrophenylhydrazine interfere they present high concentration.