MiR-346 promotes the biological function of breast cancer cells by targeting SRCIN1 and reduces chemosensitivity to docetaxel

摘要: MicroRNAs (miRNAs) are a class of highly conserved small noncoding RNAs that play pivotal roles at the post-transcriptional level in biological function various cancers, including breast cancer. In our study, miR-346 mimic, inhibitor, negative control or si-SRCIN1 were transfected into MCF-7 and MCF-7/Doc cells, respectively. Quantitative real time PCR (qRT-PCR) was used to measure SRCIN1 mRNA expressions western blot detect expression protein level. CCK-8 colony formation employed verify cell viability proliferation. Flow cytometry showed apoptosis. Transwell performed migration invasion. The luciferase reporter assay data target correlation SRCIN1. Firstly, we found higher cancer tissues than their paired corresponding non-cancerous there significant inversed between Overexpression promoted proliferation, formation, invasion, reduced apoptosis, sensitivity Docetaxel (Doc). identified as direct miR-346, whose silencing proliferation IC50 Doc. Moreover, effect down-expression. Taken together, may an oncogenic miRNA mediate chemosensitivity docetaxel through targeting cancer, targeted modulation became potential strategy for treatment.