m‐carboxycinnamic acid bishydroxamide improves developmental competence, reduces apoptosis and alters epigenetic status and gene expression pattern in cloned buffalo (Bubalus bubalis) embryos

摘要: Incomplete or aberrant reprogramming of nuclear genome is one the major problems in somatic cell transfer. In this study, we studied effect histone deacetylase inhibitor m-carboxycinnamic acid bishydroxamide (CBHA) on vitro development buffalo embryos produced by Hand-made cloning. Cloned were treated with CBHA (0, 5, 10, 20 50 μM) for 10 hr from start reconstruction till activation. At 10 μM, but not at other concentrations examined, increased (p < .05) blastocyst rate (63.77 ± 3.97% vs 48.63 ± 3.55%) and reduced apoptotic index cloned blastocysts (8.91 ± 1.94 4.36 ± 1.08) compared to untreated controls, levels similar those IVF (4.78 ± 0.74). treatment, all global level H3K9ac than controls that observed blastocysts. Treatment (10 μM) decreased H3K27me3 it was still higher treatment relative expression pluripotency-related genes OCT-4 NANOG, anti-apoptotic gene BCL-XL, pro-apoptotic BAX did affect apoptosis-related p53 CASPASE3 epigenetics-related DNMT1, DNMT3a HDAC1. These results suggest 10 μM improves rate, reduces apoptosis alters epigenetic status pattern.