Supplementation of tauroursodeoxycholic acid during IVC did not enhance in vitro development and quality of buffalo IVF embryos but combated endoplasmic reticulum stress

摘要: Endoplasmic reticulum (ER) stress, a dysfunction in protein-folding capacity of ER, is involved many pathologic and physiological responses including embryonic development. This study investigated the effect supplementation IVC medium with an ER stress inducer, tunicamycin (TM), inhibitor, tauroursodeoxycholic acid (TUDCA), on developmental competence, apoptosis, gene expression buffalo embryos produced by IVF. Treatment presumed zygotes TM resulted significant (P < 0.01) decrease blastocyst rate, whereas TUDCA did not improve development rate. Further, presence could ameliorate adverse effects terms rate combined (TM + TUDCA) treatment. Tunicamycin treatment increased apoptotic index reduced total cell number, affect them significantly. However, extent TM-mediated apoptosis during decreased level chaperones, GRP78 GRP94. In treatment, their compared to that controls. A similar pattern was observed case proapoptotic BAX. We find any difference BCl-XL, BID, P53, CASPASE 3 after supplementation. conclusion, our reported induces vitro resulting increase these actions are mediated modulating apoptosis-related genes chaperones. Tauroursodeoxycholic potential embryos; however, it attenuated TM-induced downregulating BAX