Identification of a G protein alpha subunit from Neurospora crassa that is a member of the Gi family.
作者： G.E. Turner , K.A. Borkovich
DOI: 10.1016/S0021-9258(18)82405-X
关键词: G12/G13 alpha subunits 、 Heterotrimeric G protein 、 Biochemistry 、 Neurospora crassa 、 G protein 、 Consensus sequence 、 G alpha subunit 、 Genetics 、 Neurospora 、 Biology 、 Protein subunit
摘要: Heterotrimeric G proteins, consisting of alpha, beta, and gamma subunits, are implicated in major signal transduction pathways controlling a diversity functions eukaryotic organisms. In the filamentous fungus Neurospora crassa, proteins regulation several environmental responses. As first step studying role these processes, we have cloned genes for two alpha gna-1 gna-2, from Neurospora. The located on different chromosomes differentially regulated during asexual development. encoded (Gna-1 Gna-2) same size as members Gi-alpha family (approximately 40 kDa). Gna-1 protein sequence is 55% identical overall to Gi contains consensus sequences ADP-ribosylation by pertussis toxin incorporation myristic acid, which found this group. These properties make identified microbial subunit be member any class. Furthermore, incubation N. crassa plasma membrane fraction with results substrate approximate Gna-1. predicted Gna-2 does not share high degree identity However, coding region at least one intron position conserved family. We propose that subunits ancient evolution may appeared fungi.
sci-hub.st 参考文章(41)
C J Raport, B Dere, J B Hurley, Characterization of the mouse rod transducin alpha subunit gene. Journal of Biological Chemistry. ,vol. 264, pp. 7122- 7128 ,(1989) , 10.1016/S0021-9258(18)83210-0
Rowland H. Davis, Frederick J. de Serres, [4] Genetic and microbiological research techniques for Neurospora crassa Methods in Enzymology. ,vol. 17, pp. 79- 143 ,(1970) , 10.1016/0076-6879(71)17168-6
Katherine A. Borkovich, Melvin I. Simon, Coupling of receptor function to phosphate-transfer reactions in bacterial chemotaxis. Methods in Enzymology. ,vol. 200, pp. 205- 214 ,(1991) , 10.1016/0076-6879(91)00140-R
Emma Jean Bowman, Barry J. Bowman, Purification of vacuolar membranes, mitochondria, and plasma membranes from Neurospora crassa and modes of discriminating among the different H+-ATPases. Methods in Enzymology. ,vol. 157, pp. 562- 573 ,(1988) , 10.1016/0076-6879(88)57104-5
M J Orbach, M S Sachs, C Yanofsky, The Neurospora crassa arg-2 locus. Structure and expression of the gene encoding the small subunit of arginine-specific carbamoyl phosphate synthetase. Journal of Biological Chemistry. ,vol. 265, pp. 10981- 10987 ,(1990) , 10.1016/S0021-9258(19)38545-X
R E West, J Moss, M Vaughan, T Liu, T Y Liu, Pertussis toxin-catalyzed ADP-ribosylation of transducin. Cysteine 347 is the ADP-ribose acceptor site. Journal of Biological Chemistry. ,vol. 260, pp. 14428- 14430 ,(1985) , 10.1016/S0021-9258(17)38585-X
C Sadhu, D Hoekstra, M J McEachern, S I Reed, J B Hicks, A G-protein alpha subunit from asexual Candida albicans functions in the mating signal transduction pathway of Saccharomyces cerevisiae and is regulated by the a1-alpha 2 repressor. Molecular and Cellular Biology. ,vol. 12, pp. 1977- 1985 ,(1992) , 10.1128/MCB.12.5.1977
Suki Parks, Eric Wieschaus, The drosophila gastrulation gene concertina encodes a Gα-like protein Cell. ,vol. 64, pp. 447- 458 ,(1991) , 10.1016/0092-8674(91)90652-F
M. Simon, M. Strathmann, N. Gautam, Diversity of G proteins in signal transduction. Science. ,vol. 252, pp. 802- 808 ,(1991) , 10.1126/SCIENCE.1902986
K. M. Warpeha, H. E. Hamm, M. M. Rasenick, L. S. Kaufman, A blue-light-activated GTP-binding protein in the plasma membranes of etiolated peas. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 8925- 8929 ,(1991) , 10.1073/PNAS.88.20.8925