A G-protein alpha subunit from asexual Candida albicans functions in the mating signal transduction pathway of Saccharomyces cerevisiae and is regulated by the a1-alpha 2 repressor.

摘要: We have isolated a gene, designated CAG1, from Candida albicans by using the G-protein alpha-subunit clone SCG1 of Saccharomyces cerevisiae as probe. Amino acid sequence comparison revealed that CAG1 is more homologous to than any other G protein reported so far. Homology between and not only includes conserved guanine nucleotide binding domains but also spans normally variable regions which are thought be involved in interaction with components specific signal transduction pathway. Furthermore, contains central domain, previously found SCG1. cag1 null mutants C. created gene disruption produced no readily detectable phenotype. The complemented both growth mating defects S. scg1 when carried on either low- or high-copy-number plasmid. In diploid albicans, transcript was mycelial yeast cells white opaque forms. However, CAG1-specific transformants containing observed haploid cells. This transcription pattern matches caused a1-alpha 2 mediated repression That is, behaves haploid-specific cerevisiae, subject control mating-type regulation infer these results may system analogous controlling type possibly even sexual pathway has far remained undetected.