Alterations in the DNA Metabolism of MCa-11 Mouse Mammary Tumor Cells Grown in Vivo and in Vitro

摘要: Abstract Mouse mammary carcinoma (MCa-11) cells were grown in vitro exponential, plateau-fed, and starved monolayer cultures or as 100-, 250-, 500-µm tissue culture spheroids, vivo small (4-mm diameter) large (12-mm tumors. In all of these forms, the growth rates MCa-11 found to decrease after an initial rapid proliferation a relatively number cells. The DNA distributions during different , well proportion intensity labeling S-phase with [ 3 H]thymidine H]deoxyuridine, measured by flow absorption cytometry. We that significant numbers remained S phase, even had slowed. However, decreased, labeled exogenous precursors decreased. conclude progressive alterations, including possible slowing cessation, replicative synthesis occur tumor metabolic constraints on are increased.