18 – Tissue Culture, Cell Growth, and Analysis

摘要: Tissue culture is one of the most important research tools in cell biology. supplies and work areas must be sterile. This difficult when many people are working laboratory. Access to area facility dedicated tissue should limited those who actually culturing cells. It necessary have proper laboratory environment equipment perform successfully. Ideally, physically isolated from general traffic flow All precautions employed continuously avoid contamination area, which at least three air changes per hour contain following equipment: a incubator; laminar high-efficiency particulate (HEPA)-filtered hood; centrifuge; inverted phase microscope; hemocytometer/light microscope and/or Coulter counter for counting. Also, refrigerator, autoclave, standard freezer (−4°C), ultralow (−80°C), liquid nitrogen storage system (− 130°C) long-term preservation frozen lines available.