Selective aggregation of PAMAM dendrimer nanocarriers and PAMAM/ZnPc nanodrugs on human atheromatous carotid tissues: a photodynamic therapy for atherosclerosis

摘要: Photodynamic therapy (PDT) involves the action of photons on photosensitive molecules, where atomic oxygen or OH(-) molecular species are locally released pathogenic human cells, which mainly carcinogenic, thus causing cell necrosis. The efficacy PDT depends local nanothermodynamic conditions near cell/nanodrug system that control both level intracellular translocation nanoparticles in and their agglomeration membrane. Dendrimers considered one most effective promising drug carriers because relatively low toxicity negligible activation complementary reactions. Polyamidoamine (PAMAM) dendrite delivery agents has been investigated last few years for tumour selectivity, retention, pharmacokinetics water solubility. Nevertheless, use as photosensitizing molecules cardiovascular disease, targeting selective necrosis macrophage cells responsible atheromatous plaque growth, never investigated. Furthermore, aggregation, nanodrug PAMAM dendrimers PAMAM/zinc phthalocyanine (ZnPc) conjugates tissue endothelial is still unknown. In this work, aggregation zero generation (G0) acting carriers, well conjugated G0 with a ZnPc photosensitizer, to symptomatic asymptomatic carotid tissues was by using force microscopy (AFM). For evaluation texture characteristics AFM images, statistical surface morphological fractal analytical methodologies Minkowski functionals were used. All quantities showed deposition healthy an inverse impact when comparing different features between G0/ZnPc considerably larger aggregations plaque. results highlight importance dendrimer novel platform atherosclerosis therapies.