Heterogeneous Distribution of Fetal Microchimerism in Local Breast Cancer Environment
作者： Dragos Nemescu , Ramona Gabriela Ursu , Elena Roxana Nemescu , Lucian Negura
DOI: 10.1371/JOURNAL.PONE.0147675
关键词: Testis determining factor 、 Biology 、 Fetus 、 Breast cancer 、 Pregnancy 、 Microchimerism 、 Polymerase chain reaction 、 Pathology 、 Real-time polymerase chain reaction 、 Tumor microenvironment 、 General Biochemistry, Genetics and Molecular Biology 、 General Agricultural and Biological Sciences 、 General Medicine
摘要: Fetal cells enter maternal circulation during pregnancy and persist in the woman’s body for decades, achieving a form of physiological microchimerism. These were also evidenced tumors. We investigated frequency concentration fetal microchimerism local breast cancer environment. From 19 patients with confirmed neoplasia, after surgical resection, we collected three fresh specimens from tumor core, tissue at periphery, adjacent normal tissue. The presence male DNA was analyzed quantitative PCR assay sex determining region gene (SRY) gene. In group women who had given birth to least one son, detected 100% samples tumors their periphery 64% (9 14) those tissues its carry significantly increased number SRY copies compared neighboring common (p = 0.005). median normalized SRY-signal about 77 (range, 3.2–21467) 14-fold 1.3–2690) greater respectively than addition, relative expression 5.5 times larger 1.1–389.4). found heterogeneous distribution sons, neoplasia harbors higher levels peripheral
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