Subculture method of human amniotic mesenchymal stem cells
作者： Wang Yifei , Chen Haijia , Ma Yanyan , Ge Xiaohu , Wang Xiaoyan
DOI:
关键词: Cell biology 、 Cell 、 Suspension culture 、 Cell density 、 Stem cell 、 Petri dish 、 Chemistry 、 Mesenchymal stem cell 、 Primary culture 、 Subculture (biology)
摘要: The invention relates to a subculture method of human amniotic mesenchymal stem cells. the cells comprises following steps: (1) sucking off culture liquid in dish after fusion degree cell subjected primary separation reaches 80-90%, adding PBS for washing, and then PBS; (2) tryp1E into dish, transferring CO2 case, hatching 1-2 minutes, complete medium, repeatedly blowing beating until 80-90% are not clung wall; (3) centrifuging; (4) discarding supernatant liquid, re-suspending sediment by using blowing, uniformly mixing obtain suspension, counting counter; (5) inoculating suspension adjusting density be 1.3*10 /cm , medium containing 10ng/ml EGF, case culture. By virtue cells, large quantity high-activity can obtained.
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