Amniotic stem cell culture medium and culture method thereof

作者: Zhou Xing , Liu Hanlong , Duan Zhongxin , Peng Chongjun

DOI:

关键词: ImmunogenicityAndrologyGlutathioneAmniotic stem cellsFetusHydroxyethyl cellulosePlatelet-rich plasmaStromal cellSodium citrateChemistry

摘要: The invention provides an amniotic stem cell culture medium which is prepared from the following ingredients of DMEM/F12, platelet rich plasma, dextranum, triethanolamine, glutathione, hydroxyethyl cellulose, folic acid, betamethasone, progesterone, dulcite and sodium citrate. According to disclosed by invention, a human membrane taken in vitroto extract cells; compared with method utilizing solution containing fetal calf serum mes-enchymal stromal cells, stemcell has advantages no other animal source, wide ethic limit, heterogeneous immunogenicity like.

参考文章(6)
Sun Rengang, Chen Daixiong, Liu Ruming, Zhong Jianjiang, Xiao Jianhui, Hyaluronic acid method for promoting proliferation of human amniotic membrane stem cells and application thereof ,(2016)
Wei Nie, Guangli Xiao, Guoqing Zhang, Ping Gao, Yingying Zhong, Liang Wu, Junmei Zhu, Fresh amnion preservation fluid, fresh amnion preservation method and application thereof ,(2007)
Wang Yifei, Chen Haijia, Ma Yanyan, Ge Xiaohu, Wang Xiaoyan, Feng Delong, Subculture method of human amniotic mesenchymal stem cells ,(2015)