Serum-free medium of human amniotic epithelial stem cell and culture method thereof
作者： Shi Ping , Zhao Feng , Pang Xining , Lang Hongxin
DOI:
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摘要: The invention relates to a serum-free medium of human amniotic epithelial stem cell and culture method thereof. is formed by adding epidermal growth factor, transferring, insulin, sodium selenite, alanyl-L-glutamine dipeptide, alanine, asparaginate, aspartic acid, glutamic glycine, proline serine DMEM/F12 basic medium. comprises the steps digesting 2.5g/L trypsin for membrane, obtaining filtering prepare single suspension; putting into CO2 incubator which saturated humidity volume fraction are 5% at 37 DEG C through under condition achieving amplification liquid exchange subculture condition, has characteristics cells, free other animal sources, wide in source ethical restriction.
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