Risk factors associated with extended spectrum beta-lactamase Escherichia coli (CTX-M) on dairy farms in North West England and North Wales

摘要: This study investigated the potential spread of CTX-M-14 Escherichia coli from a known ESBL E. positive farm and risk factors for presence CTX-M on dairy farms. Between November 2009 March 2010, 65 farms in North West England Wales were visited animals sampled producing ESBLs. Seventeen these to have received 'index' since 2005 (linked farms). The prevalence population linked was 58.8% (10/17; CI(95%) 32.9-81.6%) randomly selected control 35.4% (17/48; 22.2-50.5%). There no significant (p>0.05) linkage detection any or specifically index farm. Group 1 (CTX-M-15, CTX-M-55, CTX-M-1, CTX-M-32), group 2 (CTX-M-2) 9 (CTX-M-14, B, CTX-M-27) identified Molecular analysis revealed that three plasmids had similar sizes (95 kbp), replicon type (IncK) backbone genes as Logistic regression used 3rd 4th generation cephalosporin (ceftiofur, cefoperazone cefquinome) livestock last 12 months nearly 4 times more likely present (p=0.037; OR=3.93). association between use 1st 2nd cephalosporins. Several other identified, such storage slurry pit, operating an open herd policy infrequent cleaning calf feeding equipment.