Electroanalytical and spectroscopic procedures for examination of interactions between double stranded DNA and intercalating drugs

摘要: A method is presented for the electroanalytical characterization of interactions dsDNA with a drug, under conditions that both agents are dissolved in phosphate buffer solution and electroactive. Normal pulse, square wave, differential cyclic voltammetries were employed measurements drug oxidation signals at carbon electrodes. UV–Vis spectroscopy was used as non-electrochemical to support data. An anticancer C-1311 (5-diethylaminoethyl-amino-8-hydroxyimidazoacridinone), has been selected examination. pulse voltammetry particularly useful showing neither nor adsorbed electrode surface. Necessary appearance well-defined voltammetric signal (guanine peak) are: rigorous chemical biological purity cell appropriate DNA. analysis obtained results confirmed there two modes interaction between dsDNA: by intercalation electrostatically. In presence excess NaCl electrostatic deteriorate. The binding constants (K 1 K 2, respectively) number (n) nucleic base pairs (bp) (m) groups (pg) interacting one molecule have determined. For strong (intercalation) values constant, 1, binding-site size, n, equal 3.7 × 104 M−1 2.1, respectively. weak 2 m parameters 0.28 × 104 M−1 4.7. process rather slow its rate (the pseudo-first-order reaction) estimated 7 × 10−4 s−1. possibility independent determination very study.