Negative selection of thymocytes. A novel polymerase chain reaction-based molecular analysis detects requirements for macromolecular synthesis.

摘要: Self-tolerance is mainly established through clonal deletion of autoreactive T cells during thymic differentiation. The mechanisms by which achieved are poorly understood. Here we use a specific polymerase chain reaction-based system to characterize DNA fragmentation and show that after in vivo treatment neonatal mice with staphylococcus enterotoxin B, selective apoptosis V beta 8+ thymocytes occurs. This process precedes detectable as determined phenotypic analysis. Moreover, administration cycloheximide and, lesser extent, actinomycin D, inhibits B thymocytes. Thus, macromolecular synthesis requirement for negative selection.