Separating and culturing process of human amnion mesenchyme stem cell and its medical composition

摘要: The present invention is separating and culturing process of human amnion mesenchyme stem cell its medical composition. includes digesting successively with trypsin, collagenase deoxyribonuclease, filtering to prepare single suspension; in DMEM/F12 culture medium VDMEM VF12 the equal ratio containing ox embryo blood serum 10-20 vol% basic fibroblast growth factor ultimate concentration ng/ml inside a box at 37 deg.c, saturated humidity CO2 5 vol%; replacing liquid passage proliferate purify cell. has wide material source no ethnic limitation application foreground. composition may be used various kinds treatment.