Separate culture method for human amniotic mesenchymal stem cells
作者： Gu Yongquan , Liu Ying
DOI:
关键词: Human leucocyte antigen 、 Cell separation 、 CD90 、 Cell membrane 、 Mesenchymal stem cell 、 Cell biology 、 Chemistry 、 HUMAN LEUKOCYTE DIFFERENTIATION ANTIGEN 、 Endoglin 、 Cell culture
摘要: The invention discloses a separate culture method for human amniotic mesenchymal stem cells, and belongs to the technical field of biology. comprises following steps: (I) cell separation; (II) culture: inoculating single separated karyocyte into plastic bottle 75cm , culturing in 5 percent CO2 incubator at temperature 37 DEG C, wherein solution is medium special cell; replacing 24 hours later, discarding non-adherent every 2 3 days; fusing cells after grow 70 80 percent, digesting with pancreatin, performing passage; passage once days C CO2. obtained express by three kinds membrane molecules, namely, leukocyte differentiation antigen CD73, CD90 CD105, do not CD45 leucocyte HLA-DR.
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