Identification by two-dimensional gel electrophoresis of a 58-kilodalton tumor necrosis factor-inducing protein of Mycobacterium tuberculosis.

摘要: We have previously identified proteins in fractions of culture filtrate Mycobacterium tuberculosis with the capacity to induce cytokine production monocytes, by using a technique we defined as "monocyte Western blotting" (immunoblotting). In this series experiments, extended two-dimensional gel electrophoresis and novel 58-kDa protein M. which induces tumor necrosis factor human monocytes. Nitrocellulose particles bearing were used develop murine monoclonal antibodies intrasplenic immunization. The was purified preparative isoelectric focusing subjected N-terminal amino acid sequence analysis. As is mediator both pulmonary macrophage activation for intracellular killing, may play an important role immunopathogenesis mycobacterial immunity.