The spatial distribution of exposed nuclear DNA in normal, cancer, and reverse-transformed cells.

摘要: The malignant CHO-K1 cell is reverse-transformed by cAMP, regaining the phenotype of a normal fibroblast. During this reaction, much its DNA re-acquires sensitivity to hydrolysis DNase I in way characteristic Exposed forms rim about nucleus both and but not CHO-K1. Reacquisition nuclear requires an organized cytoskeleton. Sequestered families different degrees sequestration. In accordance with previous theoretical developments it proposed that (i) genes specific given differentiation state are stored rim, whereas other states sequestered within nucleus; (ii) only exposed active, their activity modulated regulatory molecules fluid medium; (iii) exposure sequestration regulated cytoskeletal protein structures; (iv) at least several types cancer defect lies genome process so sequences associated growth loci have been transferred from condition consequent loss DNA. methodology described should be generally applicable examining accessibility subsets during various physiological modulations function.