L-lactic acid production from apple pomace by sequential hydrolysis and fermentation.

摘要: Abstract The potential of apple pomace (a solid waste from cider and juice making factories) as a source sugars other compounds for fermentation was evaluated. effect the cellulase-to-solid ratio (CSR) liquor-to-solid (LSR) on kinetics glucose total monosaccharide generation studied. Mathematical models suitable reproducing predicting hydrolyzate composition were developed. When samples subjected to enzymatic hydrolysis, fructose present in raw material free monosaccharides extracted at beginning process. Using low cellulase cellobiase charges (8.5 FPU/g-solid 8.5 IU/g-solid, respectively), 79% glucan saccharified after 12 h, leading solutions containing up 43.8 g monosaccharides/L (glucose, 22.8 g/L; fructose, 14.8 g/L; xylose + mannose + galactose, 2.5 g/L; arabinose + rhamnose, 2.8 g/L). These results correspond monosaccharide/cellulase 0.06 g/FPU volumetric productivity 3.65 g monosaccharides/L h. Liquors obtained under these conditions used fermentative lactic acid production with Lactobacillus rhamnosus CECT-288, media 32.5 g/L l -lactic 6 h (volumetric productivity = 5.41 g/L h, product yield = 0.88 g/g).