VHL-regulated MiR-204 suppresses tumor growth through inhibition of LC3B-mediated autophagy in renal clear cell carcinoma.
作者： Olga Mikhaylova , Yiwen Stratton , Daniel Hall , Emily Kellner , Birgit Ehmer
DOI: 10.1016/J.CCR.2012.02.019
关键词: Clear cell 、 Cell biology 、 MAP1LC3B 、 Cell 、 Biology 、 Transplantation 、 microRNA 、 Suppressor 、 Autophagy 、 Regulator
摘要: The von Hippel-Lindau tumor-suppressor gene (VHL) is lost in most clear cell renal carcinomas (ccRCC). Here, using human ccRCC specimens, VHL-deficient cells, and xenograft models, we show that miR-204 a VHL-regulated tumor suppressor acting by inhibiting macroautophagy, with MAP1LC3B (LC3B) as direct functional target. Of note, higher grade of was correlated concomitant decrease increase LC3B levels, indicating LC3B-mediated macroautophagy necessary for RCC progression. VHL, addition to inducing endogenous miR-204, triggered the expression LC3C, an HIF-regulated paralog, suppressed growth. These data reveal function VHL tumor-suppressing regulator autophagic programs.
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Marieta I. Toma, Marianne Grosser, Alexander Herr, Daniela E. Aust, Axel Meye, Christian Hoefling, Susanne Fuessel, Daniela Wuttig, Manfred P. Wirth, Gustavo B. Baretton, Loss of heterozygosity and copy number abnormality in clear cell renal cell carcinoma discovered by high-density affymetrix 10K single nucleotide polymorphism mapping array. Neoplasia. ,vol. 10, pp. 634- 642 ,(2008) , 10.1593/NEO.08160
Koji Fukunaga, Takashi Wada, Hiroaki Matsumoto, Satoru Yoshihiro, Hideyasu Matsuyama, Katsusuke Naito, Renal cell carcinoma: Allelic loss at chromosome 9 using the fluorescent multiplex–polymerase chain reaction technique Human Pathology. ,vol. 33, pp. 910- 914 ,(2002) , 10.1053/HUPA.2002.126877
Lucy Gossage, Tim Eisen, Alterations in VHL as potential biomarkers in renal-cell carcinoma Nature Reviews Clinical Oncology. ,vol. 7, pp. 277- 288 ,(2010) , 10.1038/NRCLINONC.2010.42
M. Negrini, M. Ferracin, S. Sabbioni, C. M. Croce, MicroRNAs in human cancer: from research to therapy Journal of Cell Science. ,vol. 120, pp. 1833- 1840 ,(2007) , 10.1242/JCS.03450
David Juan, Gabriela Alexe, Travis Antes, Huiqing Liu, Anant Madabhushi, Charles Delisi, Shridhar Ganesan, Gyan Bhanot, Louis S. Liou, Identification of a MicroRNA Panel for Clear-cell Kidney Cancer Urology. ,vol. 75, pp. 835- 841 ,(2010) , 10.1016/J.UROLOGY.2009.10.033
C Nakada, K Matsuura, Y Tsukamoto, M Tanigawa, T Yoshimoto, T Narimatsu, LT Nguyen, N Hijiya, T Uchida, F Sato, H Mimata, M Seto, M Moriyama, Genome-wide microRNA expression profiling in renal cell carcinoma: significant down-regulation of miR-141 and miR-200c. The Journal of Pathology. ,vol. 216, pp. 418- 427 ,(2008) , 10.1002/PATH.2437
VI Korolchuk, CD Rubinsztein, AM Cuervo, B Ravikumar, S Sarkar, S Kaushik, DJ Klionsky, In search of an "autophagomometer". Autophagy. ,vol. 5, pp. 585- 589 ,(2009) , 10.4161/AUTO.5.5.8823
Yoni Baert, Ellen Goossens, Dorien van Saen, Liang Ning, Peter in’t Veld, Herman Tournaye, Orthotopic grafting of cryopreserved prepubertal testicular tissue: in search of a simple yet effective cryopreservation protocol. Fertility and Sterility. ,vol. 97, pp. 1152- 1157 ,(2012) , 10.1016/J.FERTNSTERT.2012.02.010
George A. Calin, Carlo M. Croce, MicroRNA signatures in human cancers Nature Reviews Cancer. ,vol. 6, pp. 857- 866 ,(2006) , 10.1038/NRC1997
Daniel Heng, Ronald Bukowski, Anti-angiogenic targets in the treatment of advanced renal cell carcinoma. Current Cancer Drug Targets. ,vol. 8, pp. 676- 682 ,(2008) , 10.2174/156800908786733450