Interaction between immobilized polyelectrolyte complex nanoparticles and human mesenchymal stromal cells.

摘要: ) by analysis for metabolic activity of hMSCs in dependence PECNP surface concentration MTS (3-[4,5-dimethylthiazol-2-yl]-5-[3-carboxymethoxyphenyl]-2-[4-sulfophenyl]2H-tetrazolium, inner salt) assay, as well cell morphology (phase contrast microscopy). Results: PECNPs ranging between ~50 nm and 150 were prepared. By varying the ratio polycations polyanions, with a slightly positive (PEC + NP) or negative − net charge obtained. The composition significantly affected activity, whereas had negligible influence. Therefore, we classified into “variant systems” featuring significant dose dependency (DEAE/CS, PEI/DS) “invariant lacking such (DEAE/DS, PEI/CS). Immunofluorescence imaging fluorescein isothiocyanate isomer I (FITC)-labeled suggested internalization remaining stable 8 days. Conclusion: Our study demonstrated that affects hMSC behavior. In particular, PEI/CS system showed biocompatibility wide range, representing suitable local drug delivery from PECNP-functionalized bone substitute materials.