Microarray analysis of differential gene expression in the liver of lean and fat chickens

摘要: Excessive adiposity has become a major drawback in meat-type chicken production. However, few studies were conducted to analyze the liver expression of genes involved pathways and mechanisms leading adiposity. A previous study performed by differential display on RNAs extracted from livers lean fat lines allowed us isolate cDNA products with putative expression. In this study, microarray resource was developed these together cDNAs or related lipid metabolism. This used gene chickens. Some found difference between animals and/or correlated adipose tissue weight. Cytochrome P450 2C45, thought play role biotransformation steroids poly-unsaturated fatty acids, more expressed chickens whereas acid synthase, stearoyl-CoA desaturase, sterol response element binding factor 1 hepatocyte nuclear 4, respectively lipogenesis its regulation, These results indicate that regulation lipogenic could key fatness ontogenesis lines.