Naofen, a novel WD40-repeat protein, mediates spontaneous and tumor necrosis factor-induced apoptosis
作者： Guo-Gang Feng , Chang Li , Lei Huang , Koji Tsunekawa , Yuko Sato
DOI: 10.1016/J.BBRC.2010.02.133
关键词: Antibody 、 Caspase 、 Tumor necrosis factor alpha 、 Messenger RNA 、 HEK 293 cells 、 WD40 repeat 、 Apoptosis 、 cDNA library 、 Molecular biology 、 Biology 、 Biophysics 、 Cell biology 、 Biochemistry
摘要: Naofen has recently been identified from the rat brain/spinal cord cDNA library as a substance reactive against an anti-shigatoxin (Stx)-2 antibody. mRNA is composed of 4620 nucleotides and encodes 1170 amino acids. contains four WD-repeat domains in its N-terminus ubiquitously distributed many tissues rat. Tumor necrosis factor (TNF)-α enhanced expression naofen HEK293 cells dose-dependent manner. Furthermore, siRNA, which predominantly knocked down mRNA, significantly reduced both TNF-α-induced caspase-3 activation apoptosis cells. Overexpression (FLAG-NF) spontaneously induced caspase -3 apoptosis, showed extremely high susceptibility to apoptosis. These results indicated that may function novel modulator activating caspase-3, promoting TNF-α-stimulated
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