Regulation of Interleukin-1β-induced Interleukin-6 Gene Expression in Human Fibroblast-like Synoviocytes by p38 Mitogen-activated Protein Kinase
作者： Keiji Miyazawa , Akio Mori , Hiroshi Miyata , Masuo Akahane , Yukiyoshi Ajisawa
关键词: MAP kinase kinase kinase 、 MAP2K7 、 Mitogen-activated protein kinase kinase 、 MAPK7 、 Molecular biology 、 Chemistry 、 MAPK14 、 Cyclin-dependent kinase 9 、 c-Raf 、 ASK1 、 Cell biology 、 Biochemistry
摘要: Involvement of p38 mitogen-activated protein (MAP) kinase in interleukin (IL)-6 gene expression human fibroblast-like synoviocytes (FLSs) was assessed. MAP constitutively expressed FLSs and activated response to IL-1beta. A pyridinylimidazole compound, SB203580, inhibited activity vivo, since the MAPKAP kinase-2 (a substrate kinase) IL-1beta-stimulated totally suppressed by it. SB203580 concentration-dependently production IL-6 FLSs. The effect dependent on de novo synthesis. significantly reduced stability mRNA without affecting rate transcription. Here, we provide evidence that is IL-1beta involved synthesis stabilizing mRNA.
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