Growth suppression of human breast cancer cells by the introduction of a wild-type p53 gene.

摘要: Mutations in the p53 gene are associated with a wide variety of human tumors, including those breast. To assess functionally role development breast cancer, we introduced either wild-type or mutant cDNA into three cancer cell lines by DNA transfection. The MDA-MB 468 and T47 D contain only single mutated copies gene, whereas status line MCF 7 remains equivocal. Following transfection, cells continued to grow unaffected both vitro vivo presence high levels expression exogenous gene. In contrast, however, an was incompatible cellular growth lines. Elevated did not alter vitro. These data strongly suggest that can function as suppressor cells. That does suppress indicates at least some tumors arise without functional inactivation mutation. may represent separate prognostic group.