Analysis of cell movement by simultaneous quantification of local membrane displacement and fluorescent intensities using Quimp2.
作者： Leonard Bosgraaf , Peter J.M. van Haastert , Till Bretschneider
DOI: 10.1002/CM.20338
关键词: Cell membrane 、 Actin 、 Dynamics (mechanics) 、 Biology 、 Fluorescence 、 Biophysics 、 Cytoskeleton 、 Cell biology 、 Displacement (vector) 、 Membrane 、 Microscope
摘要: The use of fluorescent markers in living cells has increased dramatically the recent years. quantitative analysis images requires specific software. Previously, program Quimp was launched for quantitating intensities at membrane or cortex cell. However, is not well suited to quantitate local displacement. Here we present Quimp2 that capable tracking subregions time, which enables simultaneous quantification and movement. two new tools, (i) conversion filters analyze movies obtained with fluorescent, DIC phase contrast different microscopes, (ii) a macro calculates displacement provides various options display results. used here investigate molecular mechanism cell movement by correlating dynamics concentration myosin F-actin. Cell Motil. Cytoskeleton 66: 156-165,2009. (C) 2009 Wiley-Liss, Inc.
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