Expression Measurement of Many Genes Simultaneously by Quantitative RT-PCR Using Standardized Mixtures of Competitive Templates

摘要: Progress toward complete sequencing of all human genes through the Human Genome Project has already resulted in a need for methods that allow quantitative expression measurement multiple simultaneously. It is increasingly recognized relative will provide more mechanistic information regarding cell pathophysiology than individual one by or do not direct intergene comparison. In this study, previously described reverse transcription-polymerase chain reaction were modified an effort to rapid, simple method purpose. Internal standard competitive templates (CTs) prepared each gene and combined single solution containing CTs 40 at defined concentrations another. Any subsequent dilution CT mixture did alter relationship Because same it was used a...