Loss of spr1 Expression Measurable by Quantitative RT-PCR in Human Bronchogenic Carcinoma Cell Lines

摘要: Expression of the small, proline-rich protein (spr1) squamous differentiation marker was measured in five cultured normal and 12 malignant human bronchial epithelial cell (BEC) populations by quantitative reverse transcriptase polymerase chain reaction (RT-PCR). Whereas spr1 expression quantifiable inducible all populations, carcinoma lines evaluated it neither nor inducible. Primers spanning entire coding sequence amplified full-length PCR product from genomic DNA; therefore, large deletions region were not responsible for loss measurable RT-PCR. This is first molecular genetic reported that distinguishes evaluated. Because a component crosslinked envelope forms during process, we hypothesize apparent gene disrupts mechanisms terminal squam...