Genetic diversity in fluoroquinolone and macrolide-resistant Campylobacter coli from pigs.

作者: Jan Keller , Vincent Perreten

DOI: 10.1016/J.VETMIC.2005.10.019

关键词: DNA gyraseGenotypingBiologyGeneticsCampylobacter coliPopulation23S ribosomal RNARestriction fragment length polymorphismGenotypeCampylobacterMicrobiology

摘要: The genetic diversity of 115 Campylobacter coli strains, isolated from pigs 59 geographical distant farms in Switzerland, were characterized on the basis their DNA fingerprints and resistance to macrolides fluoroquinolones. Sequence analysis showed that macrolide-resistant isolates had a point mutation 23S ribosomal RNA (rRNA) genes (A2075G) fluoroquinolone-resistant gyrase gene gyrA (C257T). One strain an additional transition gyrB (A1471C). flaA restriction fragment length polymorphism (RFLP) genotyping revealed 57% genetically different. Point mutations rRNA could be found both related isolates. Additionally, with without within individual different farms. This study ciprofloxacin erythromycin-resistant C. population present pig is not issued common ancestral clone, but strains have most likely mutated independently acquire resistances under selective pressure antibiotic.

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