Serologic monitoring of a broiler breeder flock previously affected by inclusion body hepatitis and testing of the progeny for vertical transmission of fowl adenoviruses.

摘要: The increasing number of clinical cases inclusion body hepatitis (IBH) associated with fowl adenoviruses (FAdVs) is a growing concern in different parts the world, including Canada. After an outbreak IBH 10-d-old pullet broiler breeder flock, we serologically monitored flock from 8 to 46 wk age, using agar gel precipitation test (AGPT) offered by diagnostic laboratories and FAdV group-specific enzyme-linked immunosorbemt assay (ELISA) developed earlier. In addition, tested 1-d-old progeny for possible vertical transmission when approached peak egg production performing virus isolation polymerase chain reaction (PCR) procedures on target organs. As previous studies comparing 2 tests, ELISA was more sensitive than AGPT. With ELISA, few birds had weakly positive results at all strongly 12 age until end study. This therefore practical way monitor antibodies commercial flocks. None chicks were PCR, nor isolated same tissues, indicating absence infectious progeny. lack could be due presence high antibody titers layers.