Active Site Flexibility as a Hallmark for Efficient PET Degradation by I. sakaiensis PETase.
作者： Tobias Fecker , Pablo Galaz-Davison , Felipe Engelberger , Yoshie Narui , Marcos Sotomayor
DOI: 10.1016/J.BPJ.2018.02.005
关键词: Combinatorial chemistry 、 Catalytic triad 、 Thermophile 、 Hydrolysis 、 Active site 、 Rational design 、 Residue (chemistry) 、 Biodegradation 、 Catalysis 、 Chemistry
摘要: … Size-exclusion chromatography-purified PETase was incubated in the presence or absence of 10 … variants C176A, C212A, and C176A-C212A were expressed, purified and tested for …
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