Comparison of the Baird-Parker Agar and 3M Petrifilm Staph Express Count Plate Methods for Enumeration of Staphylococcus aureus in Naturally and Artificially Contaminated Foods

摘要: The recently developed 3M Petrifilm Staph Express Count plate (PFSE) method was compared with the U.S. Food and Drug Administration Bacteriological Analytical Manual's Baird-Parker agar spread (B-P) for enumeration of Staphylococcus aureus in naturally contaminated, mechanically separated poultry (MSP; n = 92) raw milk (n 12). In addition, mozzarella Parmesan cheeses hot-smoked rainbow trout chub were surface inoculated a three-strain mixture S. aureus, stored at 5°C, periodically analyzed both methods numbers aureus. For contaminated MSP samples, PFSE yielded counts that not significantly different (P > 0.05) from obtained using B-P method. From 60% (21 35) 55% (124 226), respectively, confirmed (DNAse-positive) isolates plates identified by further testing as Corresponding identification rates forming typical c...