Surface roughness influences the protein corona formation of glycosylated nanoparticles and alter their cellular uptake.

摘要: Recently the role of protein absorption in nanoparticle drug delivery has gathered significant attention as corona can significantly decide on fate nanoparticles body. Although it is known that surface chemistry will influence amount and type bound protein, there little about effect roughness topography interaction. In this work, we show how patchy noticeably reduce adsorption proteins compared to spherical with a smooth demonstrated using six ABC triblock terpolymers based glucose, mannose galactose. To obtain nanoparticles, poly(2-D-sugar ethyl acrylate)-b-poly (n-butyl acrylate)-b-poly(4-vinyl pyridine) (PSugEA-b-PBuA-b-P4VP) was prepared by reversible addition–fragmentation chain-transfer (RAFT) polymerization assembled into patch-like appearance hydrodynamic diameter around 130–160 nm. As control, were from acrylate)-b-polystyrene (PSugEA-b-PBuA-b-PS). The displayed reduced when exposed serum-supplemented cell culture media, observed dynamic light scattering. particles, however, supported formation large corona. Additionally, an enrichment haemoglobin serum solution. albumin be dependent sugar glucose resulting highest absorption. led cellular uptake unrelated underlying sugar, which supposed help targeting specific lines. This example override any attempts target receptor expressing cells.