MicroRNA‑1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl‑terminal hydrolase CYLD expression.
作者： Jun Yin , Chengyin Weng , Jieke Ma , Fanfan Chen , Yecai Huang
关键词: Ectopic expression 、 Gene knockdown 、 Cell 、 Cell growth 、 microRNA 、 Deubiquitinating Enzyme CYLD 、 Cancer research 、 Molecular biology 、 Cell cycle 、 Biology 、 Ubiquitin Carboxyl-Terminal Hydrolase CYLD
摘要: Previous studies have demonstrated that microRNAs (miRs) are important regulators involved in various cancers, including human glioblastoma (GBM). However, the underlying mechanism of miR‑1288 remains poorly understood, and its role GBM has not been reported. The present study confirmed expression was markedly upregulated GBM. Ectopic promoted prolife-ration, colony formation anchorage‑independent growth cells. Bioinformatics analysis coupled with western blotting luciferase report assays also indicated cell proliferation by targeting ubiquitin carboxyl‑terminal hydrolase (CYLD). Knockdown CYLD reversed promotion miR‑1288‑in These results suggest miR‑1288/CYLD axis may represent a potential therapeutic target for treatment
sci-hub.se 参考文章(24)
Graham R Bignell, William Warren, Sheila Seal, Meiko Takahashi, Elizabeth Rapley, Rita Barfoot, Helen Green, Carolanne Brown, Patrick J Biggs, Sunil R Lakhani, Christopher Jones, Juliana Hansen, Edward Blair, Benedikt Hofmann, Reiner Siebert, Gwen Turner, D Gareth Evans, Connie Schrander-Stumpel, Frits A Beemer, Ans Van Den Ouweland, Dicky Halley, Bertrand Delpech, Mark G Cleveland, Irene Leigh, Jaakko Leisti, Sonja Rasmussen, Margaret R Wallace, Christiane Fenske, Piu Banerjee, Naoki Oiso, Ranbir Chaggar, Samantha Merrett, Niamh Leonard, Marcel Huber, Daniel Hohl, Pam Chapman, John Burn, Sally Swift, Anna Smith, Alan Ashworth, Michael R Stratton, None, Identification of the familial cylindromatosis tumour-suppressor gene. Nature Genetics. ,vol. 25, pp. 160- 165 ,(2000) , 10.1038/76006
Vinod Gopalan, Suja Pillai, Faeza Ebrahimi, Ali Salajegheh, Tommy C Lam, Tran K Le, Nicole Langsford, Yik‐Hong Ho, Robert A Smith, Alfred K‐Y Lam, None, Regulation of microRNA-1288 in Colorectal Cancer: Altered Expression and Its Clinicopathological Significance Molecular Carcinogenesis. ,vol. 53, ,(2014) , 10.1002/MC.21993
Jing Liu, Jinling Xu, Huining Li, Cuiyun Sun, Lin Yu, Yanyan Li, Cuijuan Shi, Xuexia Zhou, Xiuwu Bian, Yifang Ping, Yanjun Wen, Shujun Zhao, Hui Xu, Linlin Ren, Tongling An, Qian Wang, Shizhu Yu, miR-146b-5p functions as a tumor suppressor by targeting TRAF6 and predicts the prognosis of human gliomas Oncotarget. ,vol. 6, pp. 29129- 29142 ,(2015) , 10.18632/ONCOTARGET.4895
Liang Zhang, Ya Ding, Zhongyu Yuan, Junling Liu, Jian Sun, Fangyong Lei, Shu Wu, Su Li, Dongsheng Zhang, MicroRNA-500 sustains nuclear factor-κB activation and induces gastric cancer cell proliferation and resistance to apoptosis Oncotarget. ,vol. 6, pp. 2483- 2495 ,(2015) , 10.18632/ONCOTARGET.2800
Lei Song, Jie Yang, Ping Duan, Jianzhong Xu, Xiangdong Luo, Fei Luo, Zehua Zhang, Tianyong Hou, Bing Liu, Qiang Zhou, MicroRNA-24 inhibits osteosarcoma cell proliferation both in vitro and in vivo by targeting LPAATβ. Archives of Biochemistry and Biophysics. ,vol. 535, pp. 128- 135 ,(2013) , 10.1016/J.ABB.2013.04.001
Mitsuhiro Hayashi, Hirofumi Jono, Satoru Shinriki, Takuya Nakamura, Jianying Guo, Aiko Sueta, Mai Tomiguchi, Saori Fujiwara, Mutsuko Yamamoto-Ibusuki, Kei-ichi Murakami, Satoshi Yamashita, Yutaka Yamamoto, Jian-Dong Li, Hirotaka Iwase, Yukio Ando, Clinical significance of CYLD downregulation in breast cancer Breast Cancer Research and Treatment. ,vol. 143, pp. 447- 457 ,(2014) , 10.1007/S10549-013-2824-3
Kenneth J. Livak, Thomas D. Schmittgen, ANALYSIS OF RELATIVE GENE EXPRESSION DATA USING REAL-TIME QUANTITATIVE PCR AND THE 2(-DELTA DELTA C(T)) METHOD Methods. ,vol. 25, pp. 402- 408 ,(2001) , 10.1006/METH.2001.1262
E. G. Van Meir, C. G. Hadjipanayis, A. D. Norden, H. K. Shu, P. Y. Wen, J. J. Olson, Exciting New Advances in Neuro-Oncology The Avenue to a Cure for Malignant Glioma CA: A Cancer Journal for Clinicians. ,vol. 60, pp. 166- 193 ,(2010) , 10.3322/CAAC.20069
Ramin Massoumi, CYLD: a deubiquitination enzyme with multiple roles in cancer Future Oncology. ,vol. 7, pp. 285- 297 ,(2011) , 10.2217/FON.10.187
Hanchun Chen, Qiong Lu, Xifeng Fei, Likui Shen, Dongyi Jiang, Dongwei Dai, miR-22 inhibits the proliferation, motility, and invasion of human glioblastoma cells by directly targeting SIRT1 Tumor Biology. ,vol. 37, pp. 6761- 6768 ,(2016) , 10.1007/S13277-015-4575-8