Comprehensive chemical derivatization for gas chromatography–mass spectrometry-based multi-targeted profiling of the major phytohormones
作者： Claudia Birkemeyer , Ania Kolasa , Joachim Kopka
DOI: 10.1016/S0021-9673(03)00356-X
关键词: Carboxylic acid 、 Gas chromatography 、 Derivatization 、 Selected ion monitoring 、 Mass spectrometry 、 Chemistry 、 Chemical ionization 、 Gas chromatography–mass spectrometry 、 Chromatography 、 Sample preparation 、 Organic chemistry 、 Analytical chemistry 、 Biochemistry 、 General Medicine
摘要: In the present investigation we report selection of N-methyl-N-(tert.-butydimethylsilyl)trifluoroacetamide (MTBSTFA) reagent as most comprehensive derivatization protocol among 17 tested reactions covering trifluoroacetylation, pentafluorobenzylation, methylations, and trimethylsilylations. MTBSTFA allowed easy robust tert.-butyldimethylsilyl 1-aminocyclopropane-1-carboxylic acid, indole-3-acetic (+/-)-jasmonic salicylic (+/-)-abscisic meta-topolin, trans-zeatin. Detection limits analysed by selected ion monitoring quadrupole GC-MS were 0.2, 0.01, 1.0, 0.02, 0.3, 0.9 pmol injected substance, respectively. Analysis gibberellic acid A3, trans-zeatin riboside acid-beta-D-glucopyranosyl ester was best when coupled splitting extracts trimethysilylation. The optimised, validated. preparation insensitive to 2% residual water < or = 1 day storage at room temperature. final scheme highly reproducible successfully applied from approximately 300 mg (fresh mass) tobacco (Nicotiana tabacum) root Arabidopsis thaliana seedling.
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