Pullulanase expression constructs containing α-amylase promoter and leader sequences
作者： Igor A. Brikun , W. Martin Teague , Phillip J. Brumm , Larry N. Allen
DOI:
关键词: Bacillus subtilis 、 Function (biology) 、 Molecular biology 、 A-DNA 、 Recombinant DNA 、 DNA 、 Amylase 、 Pullulanase 、 Biology 、 Bacillus (shape)
摘要: Disclosed herein are DNA expression constructs containing an α-amylase promoter sequence derived from Bacillus stearothermophilus, leader and a encoding pullulanase naganoensis. Microbial hosts transformed to contain the secret function pullulanases. Also disclosed is process for making recombinant pullulanases utilizing which can be produced in subtilis.
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