Trichophyton rubrum is Inhibited by Free and Nanoparticle Encapsulated Curcumin by Induction of Nitrosative Stress after Photodynamic Activation
作者： Ludmila Matos Baltazar , Aimee E. Krausz , Ana Camila Oliveira Souza , Brandon L. Adler , Angelo Landriscina
DOI: 10.1371/JOURNAL.PONE.0120179
关键词: Mechanism of action 、 Trichophyton rubrum 、 Apoptosis 、 Curcumin 、 Microbiology 、 Cell culture 、 Molecular biology 、 Biology 、 Reactive nitrogen species 、 Photosensitizer 、 Reactive oxygen species
摘要: Antimicrobial photodynamic inhibition (aPI) utilizes radical stress generated from the excitation of a photosensitizer (PS) with light to destroy pathogens. Its use against Trichophyton rubrum, dermatophytic fungus increasing incidence and resistance, has not been well characterized. Our aim was evaluate mechanism action aPI T. rubrum using curcumin as PS in both free nanoparticle (curc-np) form. Nanocarriers stabilize allow for enhanced solubility delivery. Curcumin aPI, at optimal conditions 10 μg/mL J/cm2 blue (417 ± 5 nm), completely inhibited fungal growth (p<0.0001) via induction reactive oxygen (ROS) nitrogen species (RNS), which associated death by apoptosis. Interestingly, only scavengers RNS impeded efficacy, suggesting that acts potently nitrosative pathway. The curc-np induced greater NO• expression apoptosis cells, highlighting potential treatment skin infections.
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