作者: Jeffrey S. Batten , Benedicte Desvoyes , Yoshimi Yamamura , Karen-Beth G. Scholthof
DOI: 10.1016/J.FEBSLET.2006.04.006
关键词: Guide RNA 、 Genetics 、 RNA virus 、 Tombusviridae 、 Panicum mosaic virus 、 Nucleic acid structure 、 Gene 、 Subgenomic mRNA 、 Polyadenylation 、 Biology 、 Biophysics 、 Cell biology 、 Biochemistry 、 Molecular biology 、 Structural biology
摘要: Panicum mosaic virus (PMV) is a single-stranded positive-sense RNA in the family Tombusviridae. PMV genomic (gRNA) and subgenomic (sgRNA) are not capped or polyadenylated. We have determined that uses cap-independent mechanism of translation. A 116-nucleotide translational enhancer (TE) region on 3′-untranslated both gRNA sgRNA has been identified. The TE required for efficient translation viral proteins vitro. For mutants with compromised TE, addition cap analog, transposition cis-active to another location, restored competence 5′-proximal genes