Cap-dependent and cap-independent translation in eukaryotic systems

摘要: Unlike bacterial protein synthesis, eukaryotic synthesis has several mechanisms to initiate translation including cap-dependent initiation, re-initiation and internal initiation. While there is extensive biochemical characterization of the multiple steps in most information on other two derived from studies nucleic acid sequences that influence their efficiency. However, even best circumstances, both initiation are only 25% as efficient more commonly, 1-10% efficient. This general lack efficiency leaves open possibilities for mis-interpretation/artifacts vivo (cryptic promoters, alternate splicing) or vitro (nuclease degradation). Two examples cited author's laboratory background development a set guidelines minimize errors validate authenticity