Higher order structures of the 5'-proximal region decrease the efficiency of translation of the porcine pro-opiomelanocortin mRNA.
作者： D Chevrier , C Vézina , J Bastille , C Linard , N Sonenberg
DOI: 10.1016/S0021-9258(19)35438-9
关键词:
摘要: The SP6 polymerase/promoter system was used to synthesize porcine pro-opiomelanocortin mRNAs with nucleotide sequence deletions in the 5'- as well 3'-untranslated and coding regions. translational efficiency of mutant evaluated by cell-free translation or monitoring rate extent ribosome binding presence sparsomycin. results these experiments indicate that specific sequences 5'-untranslated regions mRNA decrease its translation. Structure mapping double-strand single-strand nucleases suggests can form stable secondary structures.
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