The influence of agr and sigmaB in growth phase dependent regulation of virulence factors in Staphylococcus aureus.

摘要: The expression of many virulence determinants in Staphylococcus aureus is tightly coordinated generally by global regulatory elements such as accessory gene regulator (agr), staphylococcal and the alternative sigma factor sigmaB. We have compared two-dimensional (2-D) protein pattern extracellular extracts wild-type cells with 2-D patterns respective mutants order to identify proteins whose amount influenced a mutation agr or sigB. In quantify changes level interesting we used Ettan-fluorescence difference gel electrophoresis technique (Amersham Biosciences). As most bacteria, was strongly regulated increased mainly stationary phase growth at high cell densities. By comparing RN6390 rsbU strain that an isogenic mutant RN6911 show about 70 spots changed mutant. To analyze role sigmaB RsbU+ (RN6390 RsbU+) derivative included this study. very similar Deltaagr/DeltarsbU (RN6911) indicating opposing effect on same genes.