Accumulation of tyrosine hydroxylase molecules during growth and development of the superior cervical ganglion.

摘要: Abstract The developmental increase in tyrosine hydroxylase (T-OH) activity the superior cervical ganglion (SCG) from mice and rats was studied using a specific antibody to enzyme. Immunotitration studies demonstrated that ontogenetic rise T-OH entirely attributable an enzyme molecule numbers, not activation of pre-existent immunotitration suggested neonatal adult molecules were immunochemically similar. Furthermore, enzymes had equivalent thermo-stabilities, implying physicochemical similarity. These observations suggest there are no structural differences between T-OH, maturation does involve appearance different species molecules. postnatal accumulation protein prevented by decentralization or pharmacologic ganglionic blockade. Consequently, release presynaptic acetycholine postsynaptic depolarization appear regulate accretion protein.