Species‐specific TaqMan probes for simultaneous identification of (Gadus morhua L.), haddock (Melanogrammus aeglefinus L.) and whiting (Merlangius merlangus L.).
作者： Martin I. Taylor , Clive Fox , Ines Rico , Ciro Rico
DOI: 10.1046/J.1471-8286.2002.00269.X
关键词: Gadus 、 Haddock 、 Multiplex 、 Fishery 、 Biology 、 Single tube 、 TaqMan 、 Merlangius merlangus 、 Whiting 、 Stock assessment
摘要: We report the development of a multiplex, single tube (TaqMan) PCR assay for identification three commercially important gadoid species, cod (Gadus morhua L.), haddock (Melanogrammus aeglefinus L.) and whiting (Merlangius merlangus L.). The unambiguously identifies known adult tissue samples, ethanol-preserved eggs from all species with greater than 98% accuracy, providing powerful tool catch independent stock assessment methods, mapping spawning sites detection commercial fraud in fishing food production industries.
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