Structural and functional stabilization of phage particles in carbohydrate matrices for bacterial biosensing

摘要: Infections associated with health care services are nowadays widespread and, to the progressive emergence of microorganisms resistant conventional chemical antibiotics, major causes morbidity and mortality. One most representative in this scenario is bacterium Pseudomonas aeruginosa, which alone responsible for ca. 13-15% all nosocomial infections. Bacteriophages have been reported as a potentially useful tool diagnosis bacterial diseases, since they specifically recognize lyse isolates thus confirming presence viable cells. In present research effort, immobilization these biological (although metabolically inert) entities was achieved via entrapment within (optimized) porous (bio)polymeric matrices alginate agar, aiming at their full structural functional stabilization. Such phage-impregnated polymeric intended future use chromogenic hydrogels sensitive color changes evolving from reaction (released) intracytoplasmatic moieties, detection kit P. aeruginosa