Transcriptional profiling reveals complex regulation of the monocyte IL-1 beta system by IL-13.

摘要: IL-4 and IL-13 are prototypic Th2 cytokines that generate an "alternatively activated" phenotype in macrophages. We used high-density oligonucleotide microarrays to investigate the transcriptional profile induced human monocytes by IL-13. After 8-h stimulation with IL-13, 142 genes were regulated (85 increased 57 decreased). The majority of these related inflammatory response innate immunity; a group lipid metabolism was also identified, clear implications for atherosclerosis. In addition characteristic markers alternatively activated macrophages, number novel IL-13-regulated seen. These included various pattern recognition receptors, such as CD1b/c/e, TLR1, C-type lectin superfamily member 6. Several components IL-1 system regulated. IL-1RI, IL-1RII, IL-1Ra all up-regulated, whereas IL-1beta-converting enzyme, caspase 1, IRAK-M down-regulated. LPS-inducible 1 enzyme activity reduced IL-13-stimulated monocytes, consequent decrease pro-IL-1beta processing. data reveal has potent effect on monocytes. IL-13-induced modulation clearly highlights tightly controlled complex levels regulation production this proinflammatory cytokine.