Isoforms of the APC tumor suppressor and their ability to inhibit cell growth and tumorigenicity

摘要: Mutation of the APC tumor suppressor gene is one earliest events in development most colorectal tumors. The encodes multiple protein isoforms through a complicated pattern expression and alternative splicing. role that each isoform plays cellular physiology unknown, although presence some these postmitotic cells suggests controlling cell growth or promoting differentiation. Three differ their amino-terminal domains were evaluated by transfer experiments using colon cancer line lacks functional APC. All three alter morphology affect elongating G1 phase cycle. conventional brain-specific suppress tumorigenic phenotype cultured cells, while 0.3 does not. In support experiments, BrdU incorporation as marker for S-phase entry occurs at higher level transiently transfected with when compared to other isoforms. colocalize microtubules dramatically reduce β-catenin activity same extent suggesting different effects on tumorigenesis may be nontranscriptional origin.